KANDUNGAN TOTAL FENOL DAN UJI AKTIVITAS ANTIOKSIDAN RUMPUT LAUT COKLAT Sargassum polycystum, Padina australis DAN Turbinaria conoides DARI PANTAI SAYANG HEULANG

Abstract

Antioxidants are needed by the body to overcome and prevent oxidative stress. Various natural ingredients native to Indonesia contain lots of antioxidants with various active ingredients, one of which is brown seaweed, namely Sargassum polycystum, Padina Australis and Turbinaria conoides. Brown seaweed contains secondary metabolites that are beneficial to health, namely alkaloids, terpenoids, steroids, tannins, saponins, and glycosides which are included in the drug and pharmaceutical industry. Phenolic compounds in brown algae have structural components that are inseparable from the cell wall and have various protective functions from UV radiation, which play a role in algae and protection against biotic factors and have therapeutic properties. The aim of this study was to determine the total phenolic content and antioxidant activity of brown seaweed Sargassum polycystum, Padina australis and Turbinaria conoides from Sayang Heulang Beach. Determination of phenolic levels was carried out using the Follin-ciocalteu method and antioxidant activity testing using the 2,2-Diphenyl-1-Pikrilhidrazil (DPPH) method. Based on the research that has been done, it can be stated that the total phenolic content of the powder Sarggassum polycystum, Padina australis and Turbinaria conoides are 3.04, 0.98 and 3.24 mg SAG / g, respectively. While the antioxidant levels have IC50 of 823.71, 417.10 and 320.07 ppm.

Keywords: Antioxydant, Padina australis, Sargassum polycystum, Turbinaria conoides