INDUKSI KALUS TANAMAN PAPRIKA (Capsicum annum L. var. grossum) MENGGUNAKAN IAA DAN BAP SECARA IN VITRO

Abstract

Paprika (Capsicum annum var. grossum) is an introduced plant that has the potential to be developed in Indonesia, and has economic value. This requires a method to propagate plants with a faster time, which can be done using tissue culture techniques (in-vitro). This study was conducted to see the effect of Indole-3-Acetic-Acid (IAA) and Benzyl Amino Purine (BAP) hormone concentration levels on callus induction of paprika leaf explants in vitro. The design of this study consisted of two treatment factors, namely the first factor of the IAA hormone with 5 levels of concentration, namely 0 (control), 1 mg / L, 2 mg / L, 3 mg / L, and 4 mg / L. The second factor of BAP hormone administration with 3 levels of concentration namely 0 (control), 2 mg / L, and 4 mg / L. The plan used in this study was a Group Randomized Design (RAK) and tested with the F test if it was significantly different, continuing to use DMRT (Duncan Multiple Range Test). There were 15 treatments, with 3 repetitions. The parameters observed are the day of appearance of the callus, the number of explants in the bottle that form the callus, and the morphology of the callus. The results showed that the formation of callus explants of paprika leaves in vitro did not occur in treatment without IAA and BAP. The concentration of the combination of growth regulators IAA and BAP affects the length of callus induction time, the percentage of explants forming calluses, and callus morphology.

Keywords: Benzyl Amino Purin (BAP), Callus Induction, Indole-3-Acetic-Acid (IAA), Peppers